ABSTRACT
Objective To establish an HPLC method for simultaneous contents determination of paeoniflorin, protocatechuic acid and chlorogenic acid in Penyanjing Capsule. Methods The chromatographic separation was performed on a SHIMADZU VP-ODS-C18 column (4.6 mm × 250 mm, 5 μm); the column temperature was maintained at 30 ℃; A gradient elution of acetonitrile-0.15% phosphoric acid aqueous solution was adopted at the flow rate of 1.0 mL/min; The UV detection wavelengths were at 231, 255, and 326 nm; The injection volume was 20 μL. Results Paeoniflorin, protocatechuic acid and chlorogenic acid could be separated efficiently with this condition. The regression equation was Y=1546.4128X+127.0756 (r=0.9999), Y=2925.8468X+2204.1076 (r=0.9998), Y=893.9045X-261.7315 (r=0.9994), respectively. Paeoniflorin, protocatechuic acid and chlorogenic acid were linear in the range of 150.054–1254.50 ng, 162.33–1352.75 ng, 11.43–95.25 ng, and the average recoveries were 97.60%, 102.09% and 98.52%. Conclusion The method is simple, convenient and accurate, which can be used to the quality control of Penyanjing Capsule.
ABSTRACT
Objective To observe the curative effect of the acute pelvic inflammatory disease through the combination of Penyanjing capsule and Miconazole. Methods Grouping the research patients into two groups with 32 patients respectively:the control group was given the Miconazole and the treating group was given the combination of Penyanjing capsule and Miconazole.Results The research group had the total effective power 100% but the control group only had 62.5% and the difference had obvious significance.Conclusion The combination of Penyanjing capsule and Miconazole has conditioning effects to the acute pelvic inflammatory disease.